As the Division III Regional Championships begin in just a few days, a new No. 1 has appeared atop the NFCA Division III Top 25 Coaches Poll. DePauw (34-2) has checked into the top spot in the last regular season poll collecting all eight first-place votes.
| Rank | Team | 2021 Record | Points | Previous |
| 1 | DePauw (8) | 34-2 | 200 | T2 |
| T2 | Texas Lutheran | 28-4 | 192 | 1 |
| Virginia Wesleyan | 37-5-1 | 192 | T2 | |
| 4 | Eastern Connecticut State | 30-2 | 176 | 6 |
| 5 | Birmingham-Southern | 29-5 | 168 | 5 |
| 6 | Moravian | 30-1 | 160 | 4 |
| 7 | East Texas Baptist | 30-5 | 152 | 7 |
| 8 | Linfield | 36-7 | 144 | 8 |
| 9 | Salisbury | 24-7 | 136 | 9 |
| 10 | The College of New Jersey | 23-3 | 128 | 14 |
| 11 | Christopher Newport | 28-12 | 115 | 11 |
| 12 | Belhaven | 30-10 | 109 | 13 |
| 13 | Berry | 25-10 | 98 | 10 |
| 14 | Rochester | 31-8 | 91 | 20 |
| 15 | Emory & Henry | 26-7 | 88 | 15 |
| 16 | St. John Fisher | 28-5 | 67 | 12 |
| 17 | Alfred | 31-9 | 44 | NR |
| 18 | Mount Union | 32-7 | 41 | 16 |
| 19 | Randolph-Macon | 14-7-1 | 34 | 17 |
| T20 | Millikin | 31-11 | 33 | RV |
| St. Thomas (Minn.) | 28-8 | 33 | NR | |
| 22 | Trine | 30-8 | 32 | 22 |
| 23 | Milwaukee School of Engineering | 33-8 | 31 | RV |
| 24 | St. Olaf | 24-6 | 30 | 21 |
| 25 | Coe | 24-13 | 28 | 19 |
Others receiving votes: Mount St. Joseph 21, Concordia (Wis.) 18, Rowan 16, York (Pa.) 11, Saint Benedict 6, Benedictine 5, Stevens Institute of Technology 3, Southern Maine 3, Tufts 2, and Wisconsin-Oshkosh 1.
Dropped out: Misericordia, Transylvania and Wartburg.